Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing     

XiaoFeng Li  Chao Li  Qin Zhou  GuoXiong Chen  PengShan Zhao 《寒旱区科学》2020,12(3):180-188

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.  相似文献   

游客-环境互动视角下旅游者目的地环境恢复性效应     

朱芳  苏勤  陶云  沈惊宏 《热带地理》2020,40(4):636-648

日常生活环境和旅游目的地环境都可以为个体提供恢复的机会，不同环境的属性特征导致不同程度的恢复。为了探究环境类型与恢复性效应之间的关系及停留时间的调节作用，文章采用问卷调查方式，利用配对样本t检验和混合设计方差分析相结合的方法，探讨游客在不同环境中的恢复性效应，并对这一现象提供心理上的解释。结果表明：1）恢复体验的4个维度（放松体验、心理脱离、掌握体验、控制体验）在旅游目的地环境中的得分均高于日常生活环境。2）掌握和控制体验维度在城市旅游目的地中的变化程度高于乡村旅游目的地，两种类型目的地的放松和心理脱离维度差异较小。3）随着在目的地停留时间的延长，乡村旅游目的地放松体验和心理脱离维度变化程度高于城市旅游目的地，两种类型目的地环境恢复性效应会相互靠近。因此，游客的环境恢复性效应不仅受到环境和目的地类型的影响，同时还与游客的适应程度有关，且受到其停留时间的调节作用。  相似文献   

Spatial variation of the relationship between transport accessibility and the level of economic development in Qinghai-Tibet Plateau,China     

YANG Xue-ting  QIU Xiao-ping  FANG Yi-ping  XU Yun  ZHU Fu-biao 《山地科学学报》2019,(8)

As an important component of China' transportation systems, for a long time, the insufficient performance of transport in QinghaiTibet Plateau(QTP) was a bottleneck restricting the economic growth and social development in this area. Nevertheless, the implementation of the western development strategy has accelerated the preliminary construction of comprehensive transport network since 2000. Due to the large area and significant geographical heterogeneity, there is a growing need to understand the relationship between transportation and economic development based on the perspective of spatial difference. By using GIS-based raster analysis and Geographically Weighted Regression(GWR) model, we investigated the spatial-temporal distribution of highway, railway and airport accessibility, respectively, and estimated the correlation and heterogeneity between transport accessibility and the level of economic development. Results revealed that:(1) Transport accessibility in the QTP improved by 53.38% in the past 15 years, which is specifically embodied in the improvement of both highway and railway.(2) Accessibility presented prominent differentiation in the space, increasing from west to east and reducing with the rise of elevation, specifically, the best accessibility area of the highway is below 4000 m above sea level, while the area with an altitude of over 4000 m has the lowest aviation time cost.(3) In general, the long weighted average time cost to critical transport facilities posed significantly negative effect on county economic growth in QTP, more positively, the adverse effect gradually weakened over time.(4) Obvious heterogeneity exists at the influence of different transport accessibility factors on the level of economic development, reflecting both in the horizontal space and altitudinal belt. Therefore, region-specific policies should be addressed for the sustainable development of transport facilities as well as economy in the west mountain areas.  相似文献   

流星暴的航天定义     

马月华  徐品新  李广宇  包纲 《天文学报》2005,46(2):158-164

定义ZHR^*以每小时穿过1000平方公里天顶截面的，能在铝质表面打出直径不小于1厘米的弹坑的流星体数，作为流星群对航天安全威胁强度的定量标志，给出了从ZHR到ZHR^*的换算公式．用ZHR^*取代ZHR来评价20世纪90年代以来的一些强流星雨，发现1998年的贾可比尼(天龙座)流星雨对航天安全来说，是比公认的1999、2001、2002年3次狮子座流星暴强数倍的更强流星暴．  相似文献   

观驾山—黑岛一带信息量法找金研究     

简文星  吕贻峰  秦松贤  刘吉平 《国土资源》1996,(2)

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的  相似文献   

Research on the effect of sludge fertilizer on farmland and the safety of heavy metals in a karst area     

Qinglin Xie  Xuehong Zhang  Dunqiu Wang  Jincheng Li  Yiyong Qin  Yudao Chen 《Environmental Geology》2001,41(3-4):352-357

  相似文献   

似刺鳊鮈肌肉营养成分与品质的评价   总被引：1，自引：0，他引：1  

顾若波  徐钢春  华丹  闻海波  王玉芳  张守领 《中国海洋大学学报(自然科学版)》2008,38(2):263-268

利用常规方法对似刺鳊鮈(Paracanthobrama guichenoti Bleeker)进行了含肉率、可食率、肌肉弹性、肌肉营养成分测定并系统评价了鱼肉品质,旨在评价似刺鳊鮈的营养价值,为似刺鳊鮈的营养生理、养殖和加工研究提供基础资料.结果显示,似刺鳊鮈雄鱼的含肉率为(72.76±0.89)%,雌鱼为(68.99±0.76)%;雄鱼可食率为(78.36±0.93)%,雌鱼为(83.75±1.01)%;肌肉弹性值为(547.86±21.48)g;鲜样中粗蛋白含量为(18.10±0.17)%,粗脂肪含量为(2.32±0.13)%,水分(78.10±0.87)%,灰分(1.07±0.12)%;肌肉含有17种氨基酸,总量占鲜样的(14.91±0.31)%,其中7种人体必需氨基酸(除色氨酸外)含量占鲜样的(6.07±0.07)%,与非必需氨基酸的比值(EAA/NEAA)为68.67%,其必需氨基酸构成比例基本符合FAO/WHO的标准.必需氨基酸指数(EAAI)为73.46,支链氨基酸与芳香族氨基酸的比值(F值)为2.21,4种鲜味氨基酸占鲜样的5.73%;脂肪酸中EPA与DHA质量分数分别为2.94%,1.26%;微量元素含量丰富,钙镁含量较高;鱼肉品质系统评价结果表明,似刺鳊鮈是一种营养价值较高且有一定保健作用的鱼类.  相似文献   

基于ArcGIS的二次调查地籍图形拓扑检测   总被引：1，自引：0，他引：1  

周兴付  徐君  武俊红 《北京测绘》2009,(3):39-41

为了保证二次调查地籍数据库的质量，提高地籍空间数据检查效率，在分析二次调查地籍建库规范的基础上，提出了应用ArcGIS对地籍图形进行拓扑检测，并通过实例加以阐述。  相似文献   

Microwave Type III Burst Group in the 1993 October 2 Flare     

Qijun Fu  Zhihai Qin  Yuying Liu  Guangli Huang 《Astrophysics and Space Science》1997,250(2):189-204

Microwave Type III bursts with positive frequency drifting rate were found by Stahli and Benz (1987) for first time. Type III events are especially interesting because they are well-known to be signatures of electron beams in coronal plasma, and they are effective means for diagnosting of source plasma. A microwave burst consisting of some microwave type III burst groups was registered at Beijing Astronomical Observatory with the 2545–2645–2840 MHz synchronous observing system. The distributions of frequency drifting rate, half power duration, and intensity for each impulse in the groups have been statistically analysed. From this analysis, some important parameters for the dynamic process in the flare are deduced and discussed.  相似文献   

绿化黄河工程土地资源促进沿黄生态环境建设     

孙玉民  许曰坤  刘丕建 《山东国土资源》2006,22(10):50-51

1998年以来，国家加大对防洪工程建设投入的同时，也对工程环境保护和建设提出了更高的要求，防洪工程绿化建设已纳入了全国生态环境建设和城市总体规划，是生态建设和经济可持续发展的战略布置。它不仅可保持工程的完整，提高工程的防护性能，为抢险提供丰富的物料，而且绿化黄河沿岸，对改善生态环境，美化黄河景点，建立沿黄景观带，发展旅游观光业具有非常现实而且深远的意义，同时也是将黄河堤防建设成为防洪保安线、抢险交通线、生态景观线的重大举措。  相似文献